iGEM 2017 INSA-UPS Toulouse: Detecting and killing V. cholerae in contaminated water

This year the iGEM Toulouse team and the INSA Lyon team have merged, leading to a single team of students from the National Institute of Applied Sciences (INSA) Toulouse, University Paul Sabatier and the INSA Lyon.

The project of the team INSA-UPS Toulouse is to purify water contaminated by the pathogenic bacteria Vibrio cholerae. Cholera is still a disease that millions of people have to deal with every day.

The INSA-UPS Toulouse iGEM team

The INSA-UPS Toulouse iGEM team

Our team intends to treat small to medium volumes of contaminated water in countries impacted by cholera. For example, this year, an outbreak of cholera occurred in Yemen with already more than
250 000 people affected. Current treatments have limitations and people are still dying from cholera, either because it is hard to detect before cases are declared, or because patients live in remote areas not easily reachable by aid services. Thus, two solutions need to be found: one to detect V. cholerae before epidemic bursts occur and one to treat water in remote areas.

We want our final device to be able to combine detection and treatment of contaminated water. Furthermore, to have a greater impact, we aim for our device to be easily used by non-qualified people so everyone can contribute to improving the quality of water. We found a solution fulfilling all these criteria using synthetic biology.

Our system relies on the following biological facts: Vibrio species, hence V. cholerae, use a specific method of intra-species communication through quorum sensing. Vibrios have a specific one, using the CAI-1 molecule which binds to its specific membrane receptor CqsS. More interestingly, each Vibrio has its own CAI-1/CqsS system. That’s why, by inserting a punctual mutation on Vibrio harveyi –a nonpathogenic Vibrio- CqsS, this bacteria becomes able to detect the V. cholerae CAI-1. Using a system of communication close to the natural one will allow a strong and reliable detection of the V. cholerae in water.

The final goal is to kill the bacteria V. cholerae. We decided to focus on newly described peptides from the immune system of crocodiles. They showed a promising effect on V. cholerae. A secretion system for this peptide is needed in order to have a specific and efficient response to V. cholerae in water. Obviously, the peptide specific to V. cholerae will also kill V. harveyi.

It was thus essential to find a fast growing, large producer able to survive the antimicrobial peptides. Our team chose Pichia pastoris as it met these criteria, with a lot of publications supporting its ability to produce a great amount of antimicrobial peptide. Therefore P. pastoris has the role of cholera killer.

Summary of the iGEM team's strategy to kill  Vibrio cholerae  by using synthetic biology

Summary of the iGEM team's strategy to kill Vibrio cholerae by using synthetic biology

The link between the two previous entities is to kill V. cholerae only upon its detection. The challenge was to find a way of communication between the prokaryotic detector and the eukaryotic killer. A previously described engineered ligand/receptor system was found in the iGEM registry. This diacetyl/ODR-10 system meets perfectly our needs. Upon detection of V. cholerae, diacetyl is produced by V. harveyi. Diacetyl is then detected by the Odr10 receptor present on P. pastoris. This cell signalling induces the activation of the pFUS promoter. Behind this promoter, the peptides can be produced by reception of the signal of presence of V. cholerae.

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iGEM 2017 Manchester: On the Mission to Save Phosphorus Reserves and Clean Water

iGEM Manchester 2017 is a team of nine students participating in iGEM, the biggest synthetic biology competition in the world. In their project, iGEM Manchester aims to solve two imminent environmental dangers, which threaten our ecosystem: eutrophication and rapid depletion of phosphorus reserves. Their solution involves designing phosphate-accumulating bacteria in order to recycle phosphorus from waste-water and polluted eutrophic rivers and lakes, thus killing two birds with one stone.


iGEM Manchester 2017 started their work in early February, by analysing assorted environmental, energetic, and medical problems to be solved in society. This is a standard procedure for each of over 300 teams participating in the competition. “We considered projects ranging from fungal bricks to synthetic amino acids, from directed evolution to plastic degradation. In the end, however, we decided to focus on phosphorus exhaustion. Phosphorus is the main ingredient of agricultural fertilizers, forming the backbone of 21st-century food supply methods,” says Maciej Słowiński, a member of the team.

In fact, phosphorus is a finite resource, 99% of the reserves of which can only be found in four countries. Its reserves are predicted to be exhausted in 50–100 years. This will deliver a serious blow to the rising world population: meeting increasing demand for food might become an impossible task. At the same time, significant amounts of phosphorus end up in rivers and lakes as agricultural waste-water, giving rise to a major environmental problem: eutrophication. New recycling methods could mitigate this issue, and soaring food needs coupled with depleting phosphorus reserves create a huge incentive to develop such methods.

iGEM Manchester Team saw it as a chance to design and engineer phosphate-accumulating bacteria, thereby potentially solving two problems with one environmentally friendly and sustainable approach. The team intends to mutate the enzyme Polyphosphate Kinase (PPK) and encapsulate it in a synthetic microcompartment within E.coli. This would allow for significantly increased accumulation and storage of phosphorus inside the engineered cell compared to existing methods. Phosphate gathered in this process could be used as an organic fertilizer on farmland.

As part of the iGEM Competition, the Manchester Team will be building a business model based on the bacteria designed. To this end, the team is speaking to experts in the water industry to determine the relevance of their project and feasibility of implementation on a large scale, as well as to understand the GMO legislation framework affecting this work. In order to reach a wider public, the team also started illustrating their project on a Wiki page.

The finals of the iGEM Competition, the Giant Jamboree, take place in Boston in November. The Giant Jamboree gathers students, academia, researchers and company representatives to celebrate synthetic biology accomplishments, feature team presentations, and hold workshops as well as social events. This is where iGEM Manchester Team will compete against other universities. “We would love to receive the gold medal at the competition. For now, however, let us focus on carrying out our experiments… and on finding sponsors. After all, without them, we will not be able to complete our project,” concluded Jessica Burns, one of the team’s members.

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Synbiobeta announces Blue Sky Bio!

Get the Chance to Win Over $500k in Prizes at SynBioBeta San Francisco 2015

The Blue Sky Bio Competition allows startups, industry, and academia a chance to submit their big ideas to win over $500k in prizes. SynBioBeta, with the support of their sponsors, established this event to help cultivate the bioeconomy by providing inspiring minds with the chance to get extra resources to help fund their projects. For more contest information as well as a full prize list and additional details please go to the event webpage.

The Blue Sky Bio Competition, will be hosted during the SynBioBeta SF 2015 Conference on Nov. 4th, 2015 at The Mission Bay Conference Center, in San Francisco, CA.