The Synthetic Yeast Genome Project, Sc2.0, aims to design and build an entirely synthetic genome powering growth of the budding yeast Saccharomyces cerevisiae. While Sc2.0 represents the world's first effort to construct a designer eukaryotic genome, it won't be the last! It is time to start that discussion... At the 4th Annual Sc2.0 & Synthetic Genomes Conference, we will focus on the international collaborative effort of the Sc2.0 Research Consortium to design and build the 16 synthetic chromosomes of the designer genome. Looking beyond Sc2.0, another emphasis of the meeting will be discussing other genome engineering efforts, CRISPRs and designer nucleases, and synthetic biology. The meeting will also feature a panel discussion on genome engineering, industry and society, as well as speakers and demonstrations from the lab automation and DNA synthesis industries and a poster session. Finally, there will be in-depth technical, gustatory and social analysis of yeast products of the liquid kind.